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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

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Articles 20 Documents

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Search results for , issue "Vol 21 No 4 (2020)" : 20 Documents clear

Deteksi Molekuler Gen Penyandi Protein Virb11 pada Brucella abortus Isolat Lokal Asal Pinrang, NTT dan Strain Vaksin Maria Gladis Bupu Maze; Didik Handijatno; Wiwik Tyasningsih; Suwarno Suwarno; Agnes Theresia Soelih Estoepangestie; Rahaju Ernawati
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potentialvirulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp.virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.

Keanekaragaman Ektoparasit pada Kelelawar Subordo Microchiroptera di Goa Jepang Bukit Plawangan, Sleman, Yogyakarta Soenarwan Hery Poerwanto; Luqman Rasyid Ridhwan; Giyantolin Giyantolin; Dhea Ginawati; Desak Putu Raka Paramitha
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Ectoparasites in bats can cause a decrease in health and can be a disease vector. Ectoparasites found in bats are generally members of the Insect Class and Arachnida Class. This study was aim to determinethe diversity of ectoparasites in bats of the Microchiroptera suborder in Jepang Cave, Plawangan Hill, Sleman, Yogyakarta and knowing various factors that influence the existence of these ectoparasites in bats. Catching bats is done by mist net, bats are anesthetized and morphometric measurements are carried out. Descriptive methods are used to identify bats and their ectoparasites and analyze the abundance of ectoparasites using prevalence and intensity. Identification of ectoparasites were carriedout in the Animal Systematic Laboratory of the Parasitology Section of the Faculty of Biology, Universitas Gadjah Mada, by using the book of ectoparasites identification. Bat species were ound include Miniopterus schreibersii and Rhinolopus pusillus. The results showed that ectoparasite species were found in the batsof the Microchiroptera Suborder from the Subclass Acarina and the Insect Class. The species of Subclass Acarina found were Periglischrus sp., Spinturnix plecotinus, Blattisocius sp., And Glycyphagus sp. Speciesof the Insect Class found include Megastrebla sp., Stylidia caudata, Basilia sp., and Brachytarsina sp. The prevalence of R. pussilus in Jepang Cave, was 66.67% and it is frequent. The ectoparasite intensity categoryof Periglischrus sp. was 0.72, meanwhile Glycyphagus sp. and Stylidia caudata were 0.06, which is very low.

In Vitro Ovine Embryo Culture: Effectiveness of TCM-199 in Combination with 17?-Estradiol Hormone and Co-Culture of FTEC Yon Soepri Ondho; Dela Ayu Lestari; Daud Samsudewa
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Objective of this research was to determine the effectiveness of various culture mediums on thecleavage to morula in in vitro embryo culture of ovine. A total of 1.064 zygotes (fertilized oocytes) were grown in three kinds of different embryo culture medium, which were Tissue Culture Medium 199 (TCM-199) (T1), TCM-199 + 17â-estradiol (T2) and TCM-199 + co-culture of Fallopian Tube Epithelial Cell (FTEC) (T3). Data were analyzed using a completely randomized design with ANOVA analysis and HonestlySignificant Difference test. The variable observed were the percentage of embryo cleavage and morula. Results showed that T3 had the highest percentage (72.45%) of the cleavage occurred in zygotes cultured in the TCM-199 + FTEC co-culture medium, as well as embryo growth to the morula stage (75.75%). Incontrast, zygotes cultured in T1 and T2 had lower cleavage and lower morula development than T3, as much as 27.38%; 47.82% and 58.15%; 66.66%, respectively. In conclusion, TCM-199 + FTEC co-culture medium is the best medium for embryo culture.

Uji Aktivitas Antioksidan Daging Buah Bisbul dan Batang Buah Naga Merah Menggunakan Metode 1,1-Diphenyl-2-Picrylhydrazin Ietje Wientarsih; Bayu Febram Prasetyo; Ahmad Kurniawan; Fikri Hanifah
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Bisbul (Diospyros discolor Willd.) is a plant of the Ebenaceae family and red dragon (Hylocereus polyrhizus) is a plant of the Cactaceae family. Bisbul and red dragon have bioactive compounds are flavonoids that act as antioxidants. The purpose of this study was measure antioxidant activity from ethanol extract of bisbul pulp and ethanol extract of red dragon fruit stem by DPPH method (1,1-diphenyl-2-picrylhydrazin). Extraction by maseration method and using 96% ethanol as solution. Antioxidant activity assay using the immersion method of DPPH measured absorption at a wavelenght 517 nm and compared with vitamin C. The result showed that the ethanol extract of bisbul pulp (IC50 46.00 ìg/mL) more strong antioxidant activity compared the ethanol extract of red dragon fruit stem (IC50 1020.96 ìg/mL).

Patogenesitas Bakteri Enterobacteriaceae pada Ikan Zebra (Danio rerio) Sebagai Hewan Model Widyaningsih Rahayu; Esti Handayani Hardi; Gina Saptiani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Enterobacteriaceae are Gram negative bacteria contain endotoxin and exotoxins which arerequirements for pathogenic bacteria and act as opportunistic pathogens. The purpose of this research was to determine the ability of Enterobacteriaceae bacteria to infect zebrafish (Danio rerio) by observing anatomical pathology, mortality, time death and cumulative time of death. The method used in thisresearch is a completely randomized design method (CRD). This research was conducted in three stages, first preparation of zebrasfish as animal model by average size 3-5cm and reach three months old. Second, bacterial cultures from 10 species of Enterobacteriaceae were Escherichia coli 1, E. coli 2, Enterobactercloacae 1, Acinetobacter calcoaceticus, Enterobacter amnigenus 1, Enterobacter amnigenus 1, Escherichia coli 3, Pantoea spp., E. cloacae 1, E. cloacae 2, E. cloacae 3, cultured in Brain Heart Infused Broth (BHIB) and later media washed with Phospate Buffer Saline (PBS) 0.45%. The third stage was bacterial infection to zebrafish using immersion method with each treatment repeated 3 times and observed for 120 hours. The results showed 10 species that used caused death (mortality), the highest mortality in fish that infected with E. coli 2 and E. cloacae 1 with a percentage of 66.67% infected by E. coli 2 and E. cloacae 1. Anatomicalpathology in the external organs and internal organs showed post-infectious symptoms. Infection occurred with mean time to death of 12-86 hours, as long as the cumulative time of fish death caused by E. coli 2, A.cinetobacter calcoaceticus and E. cloacae 1 causing death from 24 hours after infection until the end ofobservation.

Epidemiologi Kejadian Demam Berdarah Dengue di Kota Ternate, Maluku Utara Sumiati Tomia; Upik Kesumawati Hadi; Susi Soviana; Elok Budi Retnani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Dengue Hemorrhagic Fever (DHF) is an endemic disease and it’s still a health problem in Ternate City. DHF is reported to occur every year and spread throughout the Districts in the Ternate Island region. Studies with epidemiological approaches according to people, places, and times that describe theincidence of DHF within a period of 10 years need to be known as an evaluation in the control program. The variables examined in this study were gender, age, number of deaths, the incidence of DHF in the subdistrict, and endemicity status. The results showed that male families suffered more DHF than female with 507 male sufferers while 411 female sufferers. The majority of DHF in the age group of 5-14 years with 507 people. The number of deaths resulting from dengue fever cases in 10 years was 31 people. The highest DHF incidence rate was in South Ternate sub-district with 379 cases and the lowest was inTernate Island sub-district with 15 cases. Endemicity status in 2019 is endemic nine villages, sporadic 45 and seven potential villages. The conclusion of this study is the incidence of dengue hemorrhagic fever ismore in men, with the age group of 5-14 years. DHF occurs throughout the year in all districts within the Ternate City area.

Pemberian Ekstrak Oncom Hitam dan Merah Memperpanjang Siklus Estrus dan Mempertebal Endometrium Tikus Putih Hanifah Alshofa Nurul Aini; Desak Nyoman Dewi Indira Laksmi; Ni Luh Eka Setiasih; Steven Dwi Purbantoro
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

hytoestrogens are active compounds, derived from plants, which have a similar structure and function as estrogen. Phytoestrogens are commonly found in legumes. Oncom, which is assumed containing phytoestrogens, is one of the most famous legumes food from Indonesia and widely consumed daily in West Java. This study was aimed to determine the effect of oncom extract on estrous cycle, endometrium thickness, and the number of antral follicles in productive age rats (Rattus novergicus). This experimental study was using 21 three-to-four-month-old fertile female rats and divided into three groups. Group I (K) was considered as a control group without any treatment. Group II and III were treatment groups which were given black (H) and red (M) oncom extracts 0.005 g/g body weight, respectively, orally with a feeding tube for 14 days. The length of the estrous cycle was measured by performing vaginal swab with interval 12 hours after first treatment was given and during the treatment. Endometrium thickness and the number of antral follicleswere measured by collecting the organs uterus and ovary for histological purpose with paraffin method after rats were euthanized post-treatment oncom extracts for 14 days. Data were analyzed by ANOVA and continued with LSD test. The total length of estrous cycle of control group, black oncom extract group, andred oncom extract group was 107,43±3,16 hours, 141,43±15,36 hours, and 161,14±17,10 hours, respectively. The mean of endometrium thickness of control group, black oncom extract group, and red oncom extract group was 346,945±65,88 ?m, 485,740±86,69 ?m, and 533,904±78,93 ?m, respectively. The number ofantral follicles of control group, black oncom extract group, and red oncom extract group was 6,00±1,54, 8,43±2,99, and 9,14±2,72, respectively. Results showed that black and red oncom extracts had a significant effect on the length of estrous cycle and endometrium thickness in rats, yet there is no significant difference in the number of antral follicles. In summary, black and red oncom extracts had effects on the length of estrous cycle and endometrium thickness, yet there was no effect on the number of antral follicles.

Kualitas Antioksidan Senyawa Fitokimia dan Karakteristik Kimia Kulit Buah Matoa (Pometia pinnata) yang Dikeringkan Mira Andriani; Nahrowi Nahrowi; Anuraga Jayanegara; Rita Mutia; Theo Mahiseta Syahniar
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was aimed to examine the influence of drying temperature on phytochemical compounds and chemical characteristic of matoa (Pometia pinnata) peels. This study used a completely randomized design with the drying temperature treatments which was divided into P0 = freeze drying (control), P1 =50Ë%C, P2 = 60Ë%C, and P3 = 70Ë%C, each treatment was dried for 48 hours. Variables observed were phytochemical compounds, water content, ashes content, crude protein, crude fat, crude fiber, IC50, malondialdehide/MDA and phenol. This study used analysis of variance followed by Duncan Multiple Range Test when the results showed significance dierence. It was calculated through Statistical Package for Social Science (SPSS) version 21.0. The results showed that the drying temperature influenced watercontent and IC 50. The phytochemical compounds of matoa peels were observed through qualitative screening included flavonoids, saponins, and tannins. The best results on the drying treatment of matoa peels was P1 (50Ë%C) which presented 9.47±0.14% water content, 3.74±0.07% ashes content, 4.89±0.03% crude protein, 0.46±0.04% crude fat, 34.42±2.16% crude fiber, 30.92±10.25 IC50, 12.85±1.49 mg/g MDA, and 0.85±0.23% phenol.

Karaterisasi Virus Avian Influenza Subtipe H5N1 Isolat Lapang Asal Bali Untuk Kandidat Vaksin Gusti Ayu Yuniati Kencana; I Nyoman Suartha; I Made Kardena; Arini Nurhandayani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

A research on the isolation and characterization of the Avian Influenza H5N1 subtype field isolate has been carried out at the BSL-3 Laboratory of PT Sanbio Laboratories, Bogor. The aim of the study was to prepare a candidate for the H5N1 subtype Avian Influenza virus vaccine. Virus isolates were taken from field isolates from Bali. A total of seven field H5N1 AI subtypes from Bali were characterized in Bogor. The isolates were: isolate 3A, isolate 4A, isolate 9C, isolate 10 A, isolate 10 C, isolate P65, isolate P67. The passage of isolates was carried out on 9-day-old embryonic Specific Pathogenic (SPF) chicken eggs by injecting 0.1 mL of SPF isolates/eggs through the allantoic cavity. Each isolate was placed in five SPF eggs and then incubated in an incubator at 37 C and candled every day. Since day 2-4 post inoculation, embryo death has occurred. The eggs are harvested by their allantoic fluid and tested for haemagglutination test(HA/HI). The HI test results were confirmed by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR) using the front primer FPHA232_13 (ATTGGTTAYCATGCAAAYAACTCG) and the back primer BPHA232_597 (GGAAAYATAGGTRGTTGGRTTYTGATAG) The results were five of the seven isolates were positive AI subtype B5 585 - 581 The five isolates of AI subtype H5N1 were subsequently sequenced, the results were all positive for AI virus subtype H5N1 clade 2.3.2. Each field isolate was given the name A / Chicken / Bali3A / GAY / 2019; A / Chicken / Bali9C / GAY / 2019; A / Chicken / BaliA4 / GAY / 2019; A / Chicken / Bali10A / GAY / 2016 and A / Chicken / Bali10C / GAY / 2019. One A / Chicken / Bali 9C / GAY / 2016 isolate was subsequently repeated 7 times until a stable H5N1 subtype AI virus titer was obtained. The results of matching with bioinformatics turned out that A / Chicken / Bali 9C / GAY / 2016 isolates had a kinship of 98.62% with AI subtype H5N1 Banyuwangi, amounting to 98.45% with AI subtype H5N1 Lamongan, amounting to 98.10% with AI-H5N1 Lumajang, 97.58% with AI-H5N1 Kediri, 97.07% with AIH5N1 Blitar, 96.72% with AI-H5N1 Denpasar, 96.72% with AI-H5N1 Buleleng and 96.72% with AI-H5N1Sukoharjo. The conclusion is one of isolate namely A / Chicken / Bali 9C / GAY / 2019 including AI subtype H5N1 clade 2.3.2, is’t stable at passage on SPF eggs, has a kinship of 96.72% with A / duck / Sukoharjo / BBVW-1428- 9/2012, the virus content is 106.9 ELD50 so it is potential for vaccine candidates.

Struktur Histologi dan Histomorfometri Kulit Anjing Ras Kintamani Asal Bali Ni Made Ayu Kurniawati; Ni Luh Eka Setiasih; Putu Suastika
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Kintamani dog is the only Balinese breed has been recognized by Federarion of International Cynology (Fédération Cynologique Internationale). Kintamani dogs live around Sukawana Village, KintamaniDistrict, Bangli Regency, of Bali. The purpose of this study was to determine the hystologycal structure and histomorphometry of kintamani dog skin. The kintamani dog used in this study were 1-2 years old.Histological structure observed by carlzeiss teaching microscope with objective lens magnification 5, 10, 20, 40, and 100x. In this study, the hystologycal structure of kintamani dog consists of the epidermis, dermis, and hypodermis, except on planum nasale. Planum nasale of kintamani dogs do not havehypodermis. The other components found in the hystologycal structure is sebaseous gland, sweat gland, hair follicles and blood vessels. While the histomorphometry of kintamani dog skin have a different thickness depending on location of the body and gender. In this study, Kintamani dog skin is the thickest in female planum nasale with thickness from epidermis to dermis at 6437.040 ?m and the thinnest inmale stomach at 2047.378 ?m.

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I G. Made Krisna Erawan

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Home Page

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Contact Name I G. Made Krisna Erawan

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Editorial Address Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location Kota denpasar, Bali INDONESIA

Abstract

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Contact Name
I G. Made Krisna Erawan

Contact Email
krisnaerawan@unud.ac.id

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Journal Mail Official
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Editorial Address
Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA